Species-Specific Identification of Human Adenoviruses by a Multiplex PCR Assay

Identification of subgenus C adenoviruses by fiber-based multiplex PCR.

Subgenus C human adenoviruses, which include serotypes 1, 2, 5, and 6, are often associated with respiratory illness, ocular infections, gastroenteritis, and systemic infection among immunocompromised patients. To address the problems associated with the conventional typing methods, we developed a fiber-based multiplex PCR assay for simple and specific identification of adenovirus type 1, 2, 5,...

Group-specific Multiplex Pcr Identification of Canine Faecal Lactobacillus Species

A set of forty-nine Lactobacillus isolates recovered from the faecal swabs of different dog breeds viz. Pomeranian (12), Labrador (15) and mongrel/indigenous dogs (22) were subjected to Lactobacillus group-specific multiplex PCR corresponding to the 16S-23S rRNA intergenic spacer region (ISR) and its flanking 23S rRNA gene. Twenty-three (46.9%) isolates were found to be positive for Group IV; s...

Identification of the classical enterotoxin genes of Staphylococcus aureus in various foods by multiplex PCR assay

Background: An annual update of information about the prevalence of Staphylococcus aureus and staphylococcal enterotoxins (SEs) genes is required in every geographic area. Aims: This study was conducted to investigate the prevalence of the bacterium and type of associated enterotoxin genes in different food samples, using multiplex polymerase ...

Multiplex PCR assay for identification of human diarrheagenic Escherichia coli.

A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli s...

Serotype Identification of Recent Iranian Isolates of Infectious Bronchitis Virus by Type-Specific Multiplex RT-PCR